Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: Summary of the 15 antibodies used for the mass cytometry analysis.

Article Snippet: Anti-Human CD36 , 152Sm , Fluidigm , 3152007B , AB_2802106.

Techniques: Mass Cytometry

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: CyTOF single-cell analysis of surface antigens in PDHGG patient-derived cell lines. t-SNE maps showing the expression of 10 surface markers (CD31, CD34, CD63, CD36, CD29, CD90, CD140a, CD49c, CD56 and CD61) in each of the eight different PDHGG patient-derived cell lines analyzed through mass cytometry technique. The color gradient refers to the intensity of the expression of the considered marker, in a blue to red scale indicating low and high intensity respectively.

Article Snippet: Anti-Human CD36 , 152Sm , Fluidigm , 3152007B , AB_2802106.

Techniques: Single-cell Analysis, Derivative Assay, Expressing, Mass Cytometry, Marker

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: List of the cell antigens used together with the molecular function and reported expression.

Article Snippet: Anti-Human CD36 , 152Sm , Fluidigm , 3152007B , AB_2802106.

Techniques: Expressing, Migration, Mutagenesis

Journal: Cell & bioscience

Article Title: MIIP downregulation drives colorectal cancer progression through inducing peri-cancerous adipose tissue browning.

doi: 10.1186/s13578-023-01179-0

Figure Lengend Snippet: Fig. 6 Communication between beige adipocytes and MIIP downregulated CRC cells promotes tumor growth in vivo. a–c HCT116-MIIP+/− or WT cells (1 × 106 cells per mouse) were co-injected unilaterally with primary adipocytes (2.5 × 105 cells per mouse) into the back of nude mice (n = 5). Tumor growth was monitored every 3 days a. The tumors were removed (b, scale bar: 1 cm) and weighed (c) at the end of the experiment. d–e Representative images (d) and statistical analysis (e) of UCP1, CD36, FABP4, and Ki67 proteins by IHC staining and representative images of H&E staining in xenograft tumors derived from (B) (n = 5, scale bar: 50 μm, red). f Representative images of IHC staining of CD36 and Ki67 proteins and H and E staining in xenograft tumors derived from mice injected with HCT116 (MIIP+/− or WT) alone (n = 5, scale bar: 200 μm). g Body weights of mice monitored in (A) on day 22 (n = 5). h Representative images and statistical analysis of endogenous CD36 and FABP4 in clinically defined human colorectal cancer samples by IHC staining (Scale bar: 100 μm, red). Adipo: mature adipocytes. All data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: For the knockdown of human CD36, we transduced CD36 or control shRNA constructs (Origene, TR314090) into parental HCT116 cells following the manufacturer’s instructions.

Techniques: In Vivo, Injection, Immunohistochemistry, Staining, Derivative Assay

Journal: Cell & bioscience

Article Title: MIIP downregulation drives colorectal cancer progression through inducing peri-cancerous adipose tissue browning.

doi: 10.1186/s13578-023-01179-0

Figure Lengend Snippet: Fig. 7 Targeting browning or FFAs uptake enhances the anti-tumor efficacy of oxaliplatin in vivo. a Flowchart of constructing the subcutaneous tumor-bearing model and implementing combined therapy in C57B/L6 mice, using oxaliplatin (5 mg/kg), SR59230A (8 mg/kg), and SSO (20 mg/ kg) through intraperitoneal injection (i.p.). b–d Subcutaneous tumor growth curve b of CMT93 scramble or stable Miip knockdown cells (1 × 105 cells per mouse) mixed with 3T3-L1 cells (2.5 × 104 cells per mouse). Treatment (vehicle, oxaliplatin, oxaliplatin plus SR59230A or SSO) began when the volume of allografts reached approximately 150 mm3. The allograft tumors were removed (c, scale bar: 1 cm) and weighed d at the end of the experiment (n = 5). e–f Kaplan–Meier analysis of disease-free survival for gene signatures related to adipocyte browning (AZGP1/UCP1/ PPARGC1A, e and FFAs transportation and oxidation (CD36/FABP4/CPT1A, f) in COAD and READ patients. g The graphical abstract describes how abnormal MIIP expression leads to excessive secretion of AZGP1, promoting bi-directional communication between CRCs and surrounding adipose tissue, and the consequent tumor-supportive role of FFAs released from adjacent adipocytes in the TME. All data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: For the knockdown of human CD36, we transduced CD36 or control shRNA constructs (Origene, TR314090) into parental HCT116 cells following the manufacturer’s instructions.

Techniques: In Vivo, Injection, Knockdown, Expressing

Journal: Journal of cell science

Article Title: Ligand-induced growth and compaction of CD36 nanoclusters enriched in Fyn induces Fyn signaling.

doi: 10.1242/jcs.188946

Figure Lengend Snippet: Fig. 7. Schematic representation of CD36 nanoclustering, Fyn enrichment and signaling. Top panel: at steady state, CD36 molecules are organized into nanoclusters (radius ∼70 nm, black circles) that are enriched with the effector kinase Fyn. Bottom panel: multivalent ligands such as TSP-1 or SMw enhance clustering, resulting in Fyn activation. Cluster enhancement is supported by the plasma membrane ultrastructure – e.g. by lipid nanodomains and cortical F-actin.

Article Snippet: Human CD36 cDNA was obtained from Origene (OriGene Technologies).

Techniques: Activation Assay, Clinical Proteomics, Membrane

Journal: BMC cancer

Article Title: CD36 promotes vasculogenic mimicry in melanoma by mediating adhesion to the extracellular matrix.

doi: 10.1186/s12885-021-08482-4

Figure Lengend Snippet: Fig. 4 Blocking CD36:TSP-1 interactions does not inhibit VM by melanoma cells. (A) VM formation by C32 melanoma cells assessed following treatment with 150 and 1500 ng/ml TSP-1. Microscopy images are representative of VM with TSP-1 treatments (scale bar = 100 μm). Right bar graph, number of VM normalized to untreated (UNT) controls. Data are mean ± SEM from n = 3 experiments. (B) VM formation by C32 melanoma cells assessed following administration of anti-CD36 mAb (10 μg/ml) or isotype IgG1 control (10 μg/ml) prior to cell seeding for VM assay. Microscopy images are representative of VM (scale bar = 100 μm). Right bar graph, number of VM normalized to untreated (UNT) controls. Data are mean ± SEM from n = 3 experiments. (C) Left histogram, flow cytometric analysis of CD36 expression on HMVEC cells with isotype control (dotted line) and CD36 (solid line). Right bar graph, survival of HMVECs assessed without or with 1500 ng/ml TSP-1 for 6 h. Data are mean ± SEM from n = 3 experiments. (D) HMVEC angiogenesis without and with 1500 ng/ml TSP-1. Microscopy images are representative of EC angiogenesis (scale bar = 500 μm). Right bar graph is number of EC branches per well normalized to untreated (UNT) controls. Data are mean ± SEM from n = 3 experiments. *p < 0.05, t-test

Article Snippet: CD36 knockdown using siRNA CD36 targeting small interfering RNAs (siRNAs) Trilencer-27 Human siRNA duplexes at 20 μM were purchased (Origene, Maryland, USA) with three CD36 targeting siRNAs (duplex sequences: SR319610A rCrArArCrCrUrArUrUrGrGrUrCrArArGrCrCrArUrCrArGAA, SR319610B rGrGrCrCrUrGrArUrArGrArArArUrGrArUr CrUrUrArCrUCA and SR319610C rGrGrArUrUrArArAr CrCrCrArArArUrGrArArGrArArGrAAC) plus a nontargeting scrambled (SCR) siRNA used as a negative control.

Techniques: Blocking Assay, Microscopy, Control, VM Assay, Expressing

Journal: BMC cancer

Article Title: CD36 promotes vasculogenic mimicry in melanoma by mediating adhesion to the extracellular matrix.

doi: 10.1186/s12885-021-08482-4

Figure Lengend Snippet: Fig. 5 CD36 facilitates the migration of melanoma cells. (A) Left panel, quantitated data of the distance travelled by C32 melanoma cells towards 10% FBS with siCD36 (siRNA A or C) or siRNA control (Scrambled). Right panel, quantitated data of total number of invading cells normalized to control (si-SCR). Data are mean ± SEM from n = 3 experiments. ****p < 0.0001 vs si-SCR, one-way ANOVA. (B) Left confocal images are representatives taken at a distance of 100 μm from the Transwell membrane start point for si-SCR control or siCD36 (A and C). Images were captured via a 20x objective on a Zeiss LSM 700 confocal microscope. Right panel, quantitated data of the number of cells at 100 μm from the Transwell membrane start point. Data are mean ± SEM from n = 3 experiments. ** p < 0.01 vs si-SCR, one-way ANOVA

Article Snippet: CD36 knockdown using siRNA CD36 targeting small interfering RNAs (siRNAs) Trilencer-27 Human siRNA duplexes at 20 μM were purchased (Origene, Maryland, USA) with three CD36 targeting siRNAs (duplex sequences: SR319610A rCrArArCrCrUrArUrUrGrGrUrCrArArGrCrCrArUrCrArGAA, SR319610B rGrGrCrCrUrGrArUrArGrArArArUrGrArUr CrUrUrArCrUCA and SR319610C rGrGrArUrUrArArAr CrCrCrArArArUrGrArArGrArArGrAAC) plus a nontargeting scrambled (SCR) siRNA used as a negative control.

Techniques: Migration, Control, Membrane, Microscopy

Journal: BMC cancer

Article Title: CD36 promotes vasculogenic mimicry in melanoma by mediating adhesion to the extracellular matrix.

doi: 10.1186/s12885-021-08482-4

Figure Lengend Snippet: Fig. 8 Schematic concept of CD36 in VM formation and cancer progression. Solid tumor growth is underpinned by angiogenesis and VM. We hypothesize that CD36 expression by tumor ECs and the cancer cells themselves interacts with integrin-α3 to promote adhesion to the tumor ECM (particularly laminin) thus facilitating tumor vascularization (i.e. angiogenesis and VM) and cancer progression. Thrombospondin (TSP-1) is a ligand for CD36 and inhibits EC-lined angiogenesis. By contrast, VM by highly malignant melanoma cells is unperturbed when TSP-1 binds CD36. This schematic was conceptualized and crafted by CSB using Microsoft PowerPoint

Article Snippet: CD36 knockdown using siRNA CD36 targeting small interfering RNAs (siRNAs) Trilencer-27 Human siRNA duplexes at 20 μM were purchased (Origene, Maryland, USA) with three CD36 targeting siRNAs (duplex sequences: SR319610A rCrArArCrCrUrArUrUrGrGrUrCrArArGrCrCrArUrCrArGAA, SR319610B rGrGrCrCrUrGrArUrArGrArArArUrGrArUr CrUrUrArCrUCA and SR319610C rGrGrArUrUrArArAr CrCrCrArArArUrGrArArGrArArGrAAC) plus a nontargeting scrambled (SCR) siRNA used as a negative control.

Techniques: Expressing

Journal: iScience

Article Title: Targeting CD36 determines nicotine derivative NNK-induced lung adenocarcinoma carcinogenesis

doi: 10.1016/j.isci.2023.107477

Figure Lengend Snippet:

Article Snippet: CD36 shRNA: 5'-GGACCATTGGTGATGAGAAGG CAAACATG-3' , Origene , Cat# TL314090.

Techniques: Recombinant, Virus, Subcloning, Plasmid Preparation, shRNA, Software

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: Summary of the 15 antibodies used for the mass cytometry analysis.

Article Snippet: Anti-Human CD36 , 152Sm , Fluidigm , 3152007B , AB_2802106.

Techniques: Mass Cytometry