Journal: Cell & bioscience

Article Title: MIIP downregulation drives colorectal cancer progression through inducing peri-cancerous adipose tissue browning.

doi: 10.1186/s13578-023-01179-0

Figure Lengend Snippet: Fig. 6 Communication between beige adipocytes and MIIP downregulated CRC cells promotes tumor growth in vivo. a–c HCT116-MIIP+/− or WT cells (1 × 106 cells per mouse) were co-injected unilaterally with primary adipocytes (2.5 × 105 cells per mouse) into the back of nude mice (n = 5). Tumor growth was monitored every 3 days a. The tumors were removed (b, scale bar: 1 cm) and weighed (c) at the end of the experiment. d–e Representative images (d) and statistical analysis (e) of UCP1, CD36, FABP4, and Ki67 proteins by IHC staining and representative images of H&E staining in xenograft tumors derived from (B) (n = 5, scale bar: 50 μm, red). f Representative images of IHC staining of CD36 and Ki67 proteins and H and E staining in xenograft tumors derived from mice injected with HCT116 (MIIP+/− or WT) alone (n = 5, scale bar: 200 μm). g Body weights of mice monitored in (A) on day 22 (n = 5). h Representative images and statistical analysis of endogenous CD36 and FABP4 in clinically defined human colorectal cancer samples by IHC staining (Scale bar: 100 μm, red). Adipo: mature adipocytes. All data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: For the knockdown of human CD36, we transduced CD36 or control shRNA constructs (Origene, TR314090) into parental HCT116 cells following the manufacturer’s instructions.

Techniques: In Vivo, Injection, Immunohistochemistry, Staining, Derivative Assay

Journal: Cell & bioscience

Article Title: MIIP downregulation drives colorectal cancer progression through inducing peri-cancerous adipose tissue browning.

doi: 10.1186/s13578-023-01179-0

Figure Lengend Snippet: Fig. 7 Targeting browning or FFAs uptake enhances the anti-tumor efficacy of oxaliplatin in vivo. a Flowchart of constructing the subcutaneous tumor-bearing model and implementing combined therapy in C57B/L6 mice, using oxaliplatin (5 mg/kg), SR59230A (8 mg/kg), and SSO (20 mg/ kg) through intraperitoneal injection (i.p.). b–d Subcutaneous tumor growth curve b of CMT93 scramble or stable Miip knockdown cells (1 × 105 cells per mouse) mixed with 3T3-L1 cells (2.5 × 104 cells per mouse). Treatment (vehicle, oxaliplatin, oxaliplatin plus SR59230A or SSO) began when the volume of allografts reached approximately 150 mm3. The allograft tumors were removed (c, scale bar: 1 cm) and weighed d at the end of the experiment (n = 5). e–f Kaplan–Meier analysis of disease-free survival for gene signatures related to adipocyte browning (AZGP1/UCP1/ PPARGC1A, e and FFAs transportation and oxidation (CD36/FABP4/CPT1A, f) in COAD and READ patients. g The graphical abstract describes how abnormal MIIP expression leads to excessive secretion of AZGP1, promoting bi-directional communication between CRCs and surrounding adipose tissue, and the consequent tumor-supportive role of FFAs released from adjacent adipocytes in the TME. All data are presented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: For the knockdown of human CD36, we transduced CD36 or control shRNA constructs (Origene, TR314090) into parental HCT116 cells following the manufacturer’s instructions.

Techniques: In Vivo, Injection, Knockdown, Expressing

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: Summary of the 15 antibodies used for the mass cytometry analysis.

Article Snippet: Anti-Human CD36 , 152Sm , Fluidigm , 3152007B , AB_2802106.

Techniques: Mass Cytometry

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: CyTOF single-cell analysis of surface antigens in PDHGG patient-derived cell lines. t-SNE maps showing the expression of 10 surface markers (CD31, CD34, CD63, CD36, CD29, CD90, CD140a, CD49c, CD56 and CD61) in each of the eight different PDHGG patient-derived cell lines analyzed through mass cytometry technique. The color gradient refers to the intensity of the expression of the considered marker, in a blue to red scale indicating low and high intensity respectively.

Article Snippet: Anti-Human CD36 , 152Sm , Fluidigm , 3152007B , AB_2802106.

Techniques: Single-cell Analysis, Derivative Assay, Expressing, Mass Cytometry, Marker

Journal: Frontiers in Oncology

Article Title: Inter and intra-tumor heterogeneity of paediatric type diffuse high-grade gliomas revealed by single-cell mass cytometry

doi: 10.3389/fonc.2022.1016343

Figure Lengend Snippet: List of the cell antigens used together with the molecular function and reported expression.

Article Snippet: Anti-Human CD36 , 152Sm , Fluidigm , 3152007B , AB_2802106.

Techniques: Expressing, Migration, Mutagenesis

Journal: Journal of cell science

Article Title: Ligand-induced growth and compaction of CD36 nanoclusters enriched in Fyn induces Fyn signaling.

doi: 10.1242/jcs.188946

Figure Lengend Snippet: Fig. 7. Schematic representation of CD36 nanoclustering, Fyn enrichment and signaling. Top panel: at steady state, CD36 molecules are organized into nanoclusters (radius ∼70 nm, black circles) that are enriched with the effector kinase Fyn. Bottom panel: multivalent ligands such as TSP-1 or SMw enhance clustering, resulting in Fyn activation. Cluster enhancement is supported by the plasma membrane ultrastructure – e.g. by lipid nanodomains and cortical F-actin.

Article Snippet: Human CD36 cDNA was obtained from Origene (OriGene Technologies).

Techniques: Activation Assay, Clinical Proteomics, Membrane

Journal: Cell

Article Title: A blood atlas of COVID-19 defines hallmarks of disease severity and specificity

doi: 10.1016/j.cell.2022.01.012

Figure Lengend Snippet:

Article Snippet: CD36 (5-271)-155Gd , Fluidigm , Cat# 3155012B, RRID: AB_2756286.

Techniques: Mass Cytometry, Flow Cytometry, Recombinant, Staining, Selection, Antibody Labeling, Labeling, Isolation, Sample Prep, Luminex, Quantitative Proteomics, Generated, Gene Expression, Clone Assay, Marker, Expressing, Mass Spectrometry, Derivative Assay, RNA Sequencing, Sequencing, Illumina Sequencing, Software, Variant Assay

Journal: Journal of Cachexia, Sarcopenia and Muscle

Article Title: A negative feedback loop between fibroadipogenic progenitors and muscle fibres involving endothelin promotes human muscle fibrosis

doi: 10.1002/jcsm.12974

Figure Lengend Snippet: Antibody panel used for CyTOF

Article Snippet: CD36 , 152Sm , 5‐271 , Fluidigm , 3152007B.

Techniques: